Download Angiotensin Protocols by Donna H. Wang PDF

By Donna H. Wang

Michigan kingdom Univ., East Lansing. interprets wisdom into reproducible equipment than allow biomedical researchers to discover the function of angiotensin in health and wellbeing and ailment. Covers genetic manipulation of detailed genes. comprises tools for useful experiences of the renin-angiotensin approach. DNLM: Renin - Angiotensin System-physiology

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3. , et al. (1996) Chimeric mice carrying ‘regional’ targeted deletion of the angiotensin type 1A receptor gene. J. Clin. Invest. 98, 1867–1877. 4. , et al. (1998) Murine double nullizygotes of the angiotensin type 1A and 1B receptor genes duplicate severe abnormal phenotypes of angiotensin nullizygotes. J. Clin. Invest. 101, 755–760. 5. , et al. (1997) Targeting deletion of angiotensin type 1B receptor gene in the mouse. Am. J. Physiol. 272, F299–304. 6. , Labosky, P. , et al. (1995) Effects on blood pressure and exploratory behaviour of mice lacking angiotensin II type-2 receptor.

Marth, J. , Orban, P. , and Rajewsky, K. (1994) Deletion of DNA polymerase ` gene segment in T cells using cell type-specific gene targeting. Science 265, 103–106. 52 Takaya, Matsusaka, and Ichikawa 9. , Betz, U. A. , and Schwenk, F. (1996) Molecular medicine in genetically engineered animals. Conditional gene targeting. J. Clin. Invest. 98, 600–603. 10. Mortensen, R. , Conner, D. , Geisterfer, L. , and Seidman J. G. (1992) Production of homozygous mutant ES cells with a single targeting construct.

3). The STOP signal is surrounded by loxP sites. In the absence of cre-recombinase, transcripts initiating at the promoter are terminated before entering the protein coding portion of the construct. In the presence of cre-recombinase, the STOP signal is eliminated and the transgene is activated. This type of temporal control over gene activation was employed by Lakso et al. (17) to study tumorigenesis in the lens of the eye. Other examples of cre-mediated gene activation have been reported (18).

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