Download Antisense and Ribozyme Methodology: Laboratory Companion by Ian Gibson PDF
By Ian Gibson
Antisense and ribozymes have a comparatively brief but winning historical past as learn instruments in gene expression stories, and hence are regarded as having excessive strength reagents in treating viral infections and cancer.
This laboratory significant other offers specific info at the strength, benefits and boundaries of this technique. It seriously discusses capability pitfalls, offers recommendations for selecting goals and supply structures, in an effort to enable the choice of the optimal technique for reaching quickly and trustworthy experimental good fortune with any human or different organic system.
For researchers, technicians and complex graduates in experimental medication, molecular and cellphone biology.
Chapter 1 Antisense and Ribozyme method (pages 1–12):
Chapter 2 layout and Synthesis of Antisense DNA Molecules (pages 13–26):
Chapter three The layout and Synthesis of Hammerhead Ribozymes (pages 27–40):
Chapter four supply of Ribozymes and Antisense DNA Molecules into Mammalian Cells (pages 41–71):
Chapter five the long run (pages 73–76):
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Additional resources for Antisense and Ribozyme Methodology: Laboratory Companion
1993). 2 Arms of Different lengths An alternative to the conventional 'symmetric' design is the so-called 'asymmetric' design (Fig. , 1994). So far, one has not observed a reduced activity in vitro or reduced efficacy in living cells of asymmetric hammerhead ribozymes when compared with their symmetric analogues. The asymmetric design of hammerhead ribozymes has a number of specific advantages. 4 Length of Arms functional domains state of the ribozyme coincidental structural domains binding cleavage formed (RNA duplex) formed Yes (catalytically active structure complex Fig.
Curr. Opinion Biotechnol. 6: 44-9 Kronenwett R, Haas R, Sczakiel G (1996):Kinetic selectivity of complementary nucleic acids: bcr-abl-directedantisense RNA and ribozymes. J. Mol. Biol. 259: 632-44 Kuo MYP, Sharmeen L, Dinter-Gottlieb D et al. (1988): Characterization of selfcleaving RNA sequences on the genome and antigenome of human hepatitis delta virus. J. Virol. 62: 2674-9 Lieber A, Strauss M (1995):Selection of efficient cleavage sites in target RNA by using a ribozyme expression library. Mol.
Usually, the selection of antisense arms is influenced by technical considerations that include the location of the cleavable motif or the location of restriction sites. Since the antisense arms serve for substrate binding, they can be used for optimization of the kinetics of substrate binding (see above). , 1993). 2 Arms of Different lengths An alternative to the conventional 'symmetric' design is the so-called 'asymmetric' design (Fig. , 1994). So far, one has not observed a reduced activity in vitro or reduced efficacy in living cells of asymmetric hammerhead ribozymes when compared with their symmetric analogues.